Cryopreservation of in vitro-grown shoot tips of papaya (Carica papaya L.) by vitrification
نویسندگان
چکیده
Shoot tips from 4to 6-week-old in vitro grown plantlets of six different cultivars of papaya were cryopreserved by vitrification. Shoot tips were treated in a 2 ml cryo-tube filled with a solution of 2 M glycerol and 0.4 M sucrose at 25°C for 20 min, then dehydrated with 1 ml pre-cooled vitrification solution at 4°C for 60 min. Treated samples were kept in liquid nitrogen for at least one week. The recovery percentages of six cultivars’ shoot tips after vitrification were between 48% and 93%. Cryopreserved shoot tips after being warmed resumed growth within 7 days and developed shoots directly without intermediate callus formation. Prolonged storage in the liquid nitrogen up to 2 years had no affect on regeneration. This study has successfully developed a simple and effective protocol for the cryopreservation of papaya shoot tips.
منابع مشابه
In vitro organogenesis and plantlet regeneration of ( Carica
An efficient protocol was developed for micro-propagation of Indian papaya (Carica papaya L.) var. Co7. Shoot bud induction occurred on culturing epicotyl segments in MS (Murashige and Skoog, 1962) medium, followed by transfer onto shoot multiplication, elongation and rooting media. Adventitious buds were induced on MS medium supplemented with varying concentration of thidiazuron (TDZ: 0.5-10 μ...
متن کاملPreservation of In Vitro Grown Shoot Tips of Potato (Solanum tuberosum L.) by Different Methods of Cryopreservation
Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of sucrose concentration, hardening temperature and different cryopreservation methods on the survival rate of potato shoot tips after cryopreservation. Excised shoot tips of in vitro plantlets of potato cultivars, At...
متن کاملCryopreservation of in vitro-grown shoot-tips of tropical taro (Colocasia esculenta var. esculenta) by vitrification.
In vitro shoot-tips of three cultivars of tropical taro (Colocasia esculenta var. esculenta (L.) Schott) were successfully cryopreserved by vitrification. Different conditioning treatments were required for each of the cultivars, while the vitrification protocol was constant for all. For the cultivars E399 and CPUK, shoot-tips from three-month-old in vitro plants grown on solidified MS were pre...
متن کاملاستخراج، شناسایی و بررسی فعالیت آنزیم پاپائین میوه Carica papaya از ایران
Background and purpose: Papain, a proteolytic enzyme, is found in the dried and purified latex from the fruits of Carica papaya L. (Caricaceae). Papain is used in medicine as a digestive and in the debridement of necrotic tissues. It is used in pharmaceutics as a spreading agent for drugs, supplied as an ointment (10%) with urea which denatures nonviable protein matter present in lesions. ...
متن کاملIn vitro cloning of female and male Carica papaya through tips of shoots and inflorescences
Raising cultures of proliferating shoots of female and male plants of Carica papaya through shoot apices taken from mature plants was difficult because of high incidence of endogenous bacterial contamination. Whilst they were easily raised through culture of young inflorescences tips of female and male plants. There was no difference in the requirement of nutrients and growth regulators for pro...
متن کامل